General Information

  • ID:  hor003295
  • Uniprot ID:  O35417
  • Protein name:  Big dynorphin
  • Gene name:  Pdyn
  • Organism:  Mus musculus (Mouse)
  • Family:  Opioid neuropeptide precursor family
  • Source:  animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Mus (subgenus), Mus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0001515 opioid peptide activity; GO:0005184 neuropeptide hormone activity; GO:0031628 opioid receptor binding
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0007268 chemical synaptic transmission; GO:0007600 sensory perception
  • GO CC:  GO:0005576 extracellular region; GO:0005886 plasma membrane; GO:0008021 synaptic vesicle; GO:0030425 dendrite; GO:0043025 neuronal cell body; GO:0043679 axon terminus; GO:0098686 hippocampal mossy fiber to CA3 synapse; GO:0098992 neuronal dense core vesicle; GO:0099013 neuronal dense core vesicle lumen

Sequence Information

  • Sequence:  YGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVT
  • Length:  32(202-233)
  • Propeptide:  MAWSRLMLAACLLVMPSNVMADCLSLCSLCAVRIQDGPRPINPLICSLECQDLVPPSEEWETCRGFSSFLTLTVSGLRGKDDLEDEVALEEGISAHAKLLEPVLKELEKSRLLTSVPEEKFRGLSSSFGNGKESELAGADRMNDEAAQGRTVHFNEEDLRKQAKRYGGFLRKYPKRSSEMARDEDGGQDGDQVGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQENPNTYSEDLDV
  • Signal peptide:  MAWSRLMLAACLLVMPSNVMA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  memory facilitation, locomotor activation and reduction of anxiety-like behavior
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  Oprk1
  • Target Unid:  P33534
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-O35417-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor003295_AF2.pdbhor003295_ESM.pdb

Physical Information

Mass: 453782 Formula: C185H292N58O41
Absent amino acids: ACEHMS Common amino acids: R
pI: 12.25 Basic residues: 10
Polar residues: 8 Hydrophobic residues: 10
Hydrophobicity: -97.81 Boman Index: -9822
Half-Life: 2.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: 2 min Aliphatic Index 66.88
Instability Index: 5977.81 Extinction Coefficient cystines: 8480
Absorbance 280nm: 273.55

Literature

  • PubMed ID:  16292317
  • Title:  Big Dynorphin, a Prodynorphin-Derived Peptide Produces NMDA Receptor-Mediated Effects on Memory, Anxiolytic-Like and Locomotor Behavior in Mice